The bands of the ladder each contain from 1 to 12 repeats of a 1018. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Invitrogen 1kb dna extension ladder 100ug nucleic acid. The new gel loading dye, purple 6x lane 1 included in the quickload purple 1 kb dna ladder. How to dilute dna ladders andersen lab, 2016 we currently have two different kinds of ladder in the lab. Dna marker bp 1 kb blue is designed for use as molecular weight standard for agarose gel electrophoresis availibility of sample size may be limited.
How can i prepare working solution with 1 kb dna ladder supplied by promega. A reference band at 1,500 bp is included for easy orientation. Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular. Limited product warranty life technologies corporation andor it affiliates warrant their products as set forth in the life technologies general terms and conditions. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. How can i prepare working solution with 1 kb dna ladder.
This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. View more information about our dna stains technology. Life technologies corporation andor its affiliates warrant their products as. Use the 1 kb plus dna ladder is to size linear doublestranded dna fragments from 100 bp to 12 kb. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. For ideal results, please use new agarose gel and change electrophoresis. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Available on gsava contract for federal government customers. How to dilute dna ladders andersen lab, 2016 invitrogen 1. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Catalog number size important guidelines 10787018 250 g.
Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and. Contents amount storage sm31 generuler 1 kb plus dna ladder 250 5 x 50 g for 500 applications, 0. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Invitrogen trackit 1 kb plus dna ladder 100 applications. The 1 kb and 3 kb bands contain more dna to provide internal orientation. Plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb. There have been no changes to product formulation or specifications. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. The ladder consists of nine bands in even increments 0. Highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes.
Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen. The invitrogen 1kb plus dna ladder is designed for sizing and quantification of double stranded dna on 0. Neb offers a variety of dna ladders with sizes ranging from 10 bp to 48. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need. The dna ladder consists of proprietary plasmids which are. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments. Tridye 1 kb plus dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The ladder is premixed with gel load buffer color dyes with varying mobility on an agarose gel for direct transfer to the gel. Dna marker can be visualized using ethidium bromide under uv light. The key to accurate band analysis is to use the correct dna ladder for your particular application. Suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb.
Formation of diffused bands of small dna fragments is a feature of agarose gel electrophoresis. Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas number mixture, not applicable ec number mixture, not applicable relevant identified uses this product is a laboratory preparation for. Shop online for a wide selection of invitrogen 1 kb plus dna ladder powerful tool for estimating molecular weight of linear, doublestranded dna fragments. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. Invitrogen egel dna ladders are formulated specifically for maximum performance on invitrogen egel precast agarose gels and are provided in readytoload formats. The 1 kb dna ladder is a readytoload molecular weight marker suitable for dna fragment size determination on gel electrophoresis. If samples need to be diluted, use te or other buffer of minimal ionic strength. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis.
For the convenience, the amounts of the 500, 1kbp and 5kbp fragments are adjusted to show greater intensities. The 1 kb dna ladder is a unique combination of a number of. This marker is ideal for the size determination of digested dna. This marker can be used with either agarose or polyacrylamide gels. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. This product is available in quickload purple format enjoy the convenience of a readytoload format. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments ranging in size from 100 bp to 15,000 bp. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm. Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular weight more 100bp and 1 kb ladders are offered from our partner biovision.
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